Swayam Central

Principles Of Downstream Techniques In Bioprocess

By Prof. Mukesh Doble   |   IIT Madras
The course covers the fundamentals, and design concepts of various down stream purification steps (unit operations) involved in a biochemical process. Down stream process is required to take a crude product from a fermentor or a bioreactor and purify it to the desired level. Hence it may involve solids, liquid and gas processing.The course covers cell breakage and recovery of intracellular material, Isolation of solids, Product recovery, Product enrichment/purification, Product polishing and finishing. This course is suitable for students pursuing their biotechnology, bioprocess engineering or other allied fieldThis course is also suitable for chemical engineers who would like to learn about separation techniques in biotechnology industries. The course will consist of lectures and solving problems. Problems will relate to design, estimating operating conditions and optimization of the process.

PREREQUISITES :Basics of biochemical engineering and thermodynamics

Learners enrolled: 434


Course Status : Upcoming
Course Type : Elective
Duration : 12 weeks
Start Date : 20 Jul 2020
End Date : 09 Oct 2020
Exam Date : 17 Oct 2020
Enrollment Ends : 27 Jul 2020
Category :
  • Biological Sciences & Bioengineering
  • Bioprocesses
  • Level : Undergraduate/Postgraduate
    This is an AICTE approved FDP course


    Week 1 : Lec-01 Introduction
    Lec-02 Mass balance, Heat Balance, flow sheet
    Lec-03 Costing
    Week 2 : Lec-04 Costing (continued), Physical and chemical principles in Downs stream
    Lec-05 Problems in Mass balance, flow sheet
    Lec-06 Cell Breakage
    Week 3 : Lec-07 Cell Breakage (continued)
    Lec-08 Solid Liquid Separation
    Lec-09 Solid Liquid Separation (continued)
    Week 4 : Lec-10 Solid Liquid separation-problems
    Lec-11 Pre-treatment and Filters
    Lec-12 Adsorption
    Week 5 : Lec-13 Adsorption (continued)
    Lec-14 Adsorption (continued)
    Lec15 Adsorption (continued)
    Week 6 : Lec-16 Liquid-Liquid Extraction
    Lec-17 Liquid-Liquid extraction (continued)
    Lec-18 Liquid-Liquid extraction (continued)
    Week 7 : Lec-19 Liquid-Liquid extraction (continued)
    Lec-20 Reversed micellar and aqueous two phase extraction
    Lec-21 Membranes
    Week 8 : Lec-22 Membranes (continued)
    Lec-23 Membranes (continued)
    Lec-24 Membranes (continued)
    Week 9 : Lec-25 Precipitation
    Lec-26 Chromatography
    Lec-27 Chromatography (continued)
    Week 10 : Lec-28 Chromatography (continued)
     Lec-29 Chromatography (continued)
     Lec-30 Chromatography (continued)
    Week 11:  Lec-31 Chromatography (continued)
     Lec-32 Chromatography (continued)
     Lec-33 Crystallisation
    Week 12 : Lec-34 Drying
     Lec-35 Drying and Distillation
     Lec-36  Future trends, Summary of the course


    1.Belter, P.A. and Cussler, E.L. Hu, W.S (1988), Bioseparation: Downstream processing for Biotechnology, Wiley, New York.
    2.Ladisch, M.R., (2001), Bioseparation Engineering: Principles, Practice and Economics, Wiley, Interscience.


    Prof. Mukesh Doble

    IIT Madras
    Mukesh Doble: Professor at the department of Biotechnology at IIT Madras. Has previously worked in Imperial chemical Industries(ICI) and General Electric(GE) for 20 years . Areas of research are Biomaterials, Biopolymers and Drug design. Published 270 papers and 10 books and filed 10 patents (including two US). Has delivered on line video courses in Downstream processes and Biostatistics.


    The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
    The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
    Date and Time of Exams: 17 October 2020 Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
    Registration url: Announcements will be made when the registration form is open for registrations.
    The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
    Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.


    Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
    Exam score = 75% of the proctored certification exam score out of 100

    Final score = Average assignment score + Exam score

    YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75. If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.

    Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Madras .It will be e-verifiable at nptel.ac.in/noc.

    Only the e-certificate will be made available. Hard copies will not be dispatched.

    Once again, thanks for your interest in our online courses and certification. Happy learning.

    - NPTEL team