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Swayam Central

Experimental Biotechnology

By Prof. Vishal Trivedi   |   IIT Guwahati
In the current MOOCs course I have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques. By the end of this course, student will be able to understand:
1. Basics of Good Lab practices.
2. Understanding different analytical techniques and their applications.
3. Specific Scientific questions and their solutions.
4. Designing new experiments.

INTENDED AUDIENCE
PG/PhD
PREREQUISITES : General Biology 10+2
INDUSTRIES  SUPPORT     : Biocon https://www.biocon.com/
Jubilant Life Sciences www.jubl.com/
Shantha Biotechnics Ltd
Panacea Biotec
Other companies related to biotechnology

Learners enrolled: 3423

SUMMARY

Course Status : Upcoming
Course Type : Elective
Duration : 12 weeks
Start Date : 14 Sep 2020
End Date : 04 Dec 2020
Exam Date : 20 Dec 2020
Enrollment Ends : 21 Sep 2020
Category :
  • Biological Sciences & Bioengineering
  • Bioprocesses
  • Biosciences
  • Level : Postgraduate
    This is an AICTE approved FDP course

    COURSE LAYOUT

    Week 1: Basics of Laboratory Research:Good Lab Practices, Concept of buffering and Preparation of Solutions and Reagents
    Week 2: Electrophoresis (Part 1):Basic Concept of Electrophoresis, performance of electrophoresis and its applications
    Week 3: Electrophoresis:Horizontal Gel electrophoresis, discussion about scientific questions and related experiments to solve them utilizing electrophoresis
    Week 4: Chromatography (Part I): Analytical techniques in purification of biomolecules; Column chromatography, HPLC.
    Week 5: Chromatography (Part II) : Analytical techniques in purification of biomolecules; Column chromatography, HPLC.
    Week 6: Chromatography (Part III) : Discussion about scientific questions and related experiments to solve them utilizing chromatography
    Week 7: Immunology Techniques: Immunological tools, Antibody Generation and Purification, ELISA, Radial Immuno-diffusion, Western blotting and Immuno-precipitation.
    Week 8: Cell Biology Techniques (Part 1): Basics of cell-culture, isolation of cells from tissue and fractionation. 
    Week 9: Cell Biology Techniques (Part 2): Immuno-localization, cell sorting, and discussion about scientific questions and related experiments to solve them utilizing cell biology techniques.
    Week 10: Molecular Biology (Part 1):Primer designing, Polymerase chain reaction, Different variants of PCR and their applications.
    Week 11:  Molecular Biology (Part 2) Blotting Techniques, DNA and protein sequencing techniques and their applications
    Week 12: Summary and Conclusions

    BOOKS AND REFERENCES

    1. H-P Schmauder, M Schweizer and L M Schweizer., Methods in Biotechnology, (eds), Taylor & Francis Publishers, 2002.
    2. K. Wilson & J. Walker, Practical Biochemistry: Principles and Techniques. (eds) Cambridge University Press, New York, 1995.
    3. Douglas A. Skoog and James J. Leary, Principles of Instrumental Analysis. 4th Edition. Saunders College Publishing, 1992.
    4. C. R. Kothari, Research methodology: Methods and Techniques, 3rd Edn., New age International 2014.
    5. R. Kumar, Research methodology a step-by-step guide for beginners, Sage Publications, London, 2011.
    6. C.G. Thomas, Research methodology and scientific writing, Ane books, Delhi, 2015.

    INSTRUCTOR BIO

    Prof. Vishal Trivedi

    IIT Guwahati
    Prof. Trivedi did his Ph.D. from Central Drug Research Institute, Lucknow in the field of Structural Biology. From his postdoctoral research at the Department of Molecular and Cellular Biology, Harvard University and Molecular Oncology Research Institute, Tufts University, Boston, USA, he gained extensive research experience in the field of cell biology, intracellular signal transduction, and immunology. Currently, his laboratory at Department of Biosciences and Bioengineering has an active group working and exploring questions related to malaria parasite biochemistry, the role of novel proteins, development of anti-malarial agents, and lastly understanding factors playing a crucial role in immunomodulation and host pathology in different organs

    COURSE CERTIFICATE

    •The course is free to enroll and learn from. But if you want a certificate, you have to register and write the proctored exam conducted by us in person at any of the designated exam centres.
    •The exam is optional for a fee of Rs 1000/- (Rupees one thousand only).
    Date and Time of Exams: 20 December 2020, Morning session 9am to 12 noon; Afternoon Session 2pm to 5pm.
    •Registration url: Announcements will be made when the registration form is open for registrations.
    • The online registration form has to be filled and the certification exam fee needs to be paid. More details will be made available when the exam registration form is published. If there are any changes, it will be mentioned then.
    •Please check the form for more details on the cities where the exams will be held, the conditions you agree to when you fill the form etc.

    CRITERIA TO GET A CERTIFICATE:
    • Average assignment score = 25% of average of best 8 assignments out of the total 12 assignments given in the course.
    • Exam score = 75% of the proctored certification exam score out of 100
    •Final score = Average assignment score + Exam score

    YOU WILL BE ELIGIBLE FOR A CERTIFICATE ONLY IF AVERAGE ASSIGNMENT SCORE >=10/25 AND EXAM SCORE >= 30/75.
    •If one of the 2 criteria is not met, you will not get the certificate even if the Final score >= 40/100.
    • Certificate will have your name, photograph and the score in the final exam with the breakup.It will have the logos of NPTEL and IIT Guwahati. It will be e-verifiable at nptel.ac.in/noc.
    •Only the e-certificate will be made available. Hard copies will not be dispatched.

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