Week 1:Introduction to Metabolic Engineering, Basic concepts; Scopes and Applications; Metabolism overview _1 (Cellular Transport processes, Fueling Reactions)
Week 2:Cellular Metabolism Overview_2 (Biosynthetic reactions, Polymerization, Growth Energetics); Regulation of Metabolic Pathways
Week 3: Reconstruction of Genome-scale metabolic network
Week 4:Examples of pathway manipulations by metabolic engineering : Ethanol, Aminoacids, antibiotics, vitamines, biopolymers, etc.
Week 5:Examples of pathway manipulations by metabolic engineering : Improvements of cellular properties, Biodegradation,
Week 6:Metabolic Flux Analysis: Flux Balance Analysis (FBA), Flux Variability Analysis, Flux Map
Week 7: Experimental determination of Metabolic Fluxes: Isotope labeled substrate, Isotope mapping Mapping Matrix, Isotope Distribution Vector
Week 8:Application of metabolic Flux Analysis
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