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Principles of BioTechnology

By Dr.P.Nagarajan   |   Tamilnadu Agricultural University, Tamil Nadu
Learners enrolled: 1654
This course is intended to the undergraduate students of any life Science discipline and those individuals who are all interested in knowing the fundamentals of biotechnology can also take up this course which will help them in doing their specialization in biotechnology in the near future. In this particular course on “Principles of Biotechnology” we are going to familiarise with the basic knowledge of Tissue culture, Molecular biology, rDNA technology, plant transformation and Immunotechnology in five subthemes.Many an occasion, new discoveries in biology have refashioned the trends of thoughts and comprehension. Such landmarks, contributed by several brilliant minds, need to be known particularly in the context of the beginning and growth of biotechnology and so this course starts with the chapter of revisiting the history and concepts of biotechnology. Well the success in understanding of biotechnology principles lies with the awareness of the in vitro culturing of plants through tissue culture principles and techniques, hence the first sub theme of this course makes you to understand the nutritional requirements of TC, the concepts of morphogenesis and the basic types of tissue culture techniques.The second sub theme basics of molecular biology was designed  in such a way to give you an over all understanding of the Nucleic Acid structure,  DNA processes,  the basics of a gene and the DNA sequencing methods. The third sub theme is rDNA technology and in which we are going to explore the fundamentals of recombinant DNA,  Examining the vector elements of DNA,  Manipulating DNA enzymes and the construction of recombinant DNA molecules. Fourth sub theme is the Plant Transformation dealts with Designing plant transformation, Familiarising with reporter genes and promoters,  Analysing methods of direct gene transfer and  indirect gene transfer. In fifth sub theme Immunotechnology introduce you to the  structure of antibodies,  Identifying the structure of an antigen,  comparing antibody and antigen, Familiarising with Monoclonal and Polyclonal antibodies and Applying Hybridoma technology.On the outset, no doubt that this course content is very well prepared to suit with the purpose of familiarising biotechnology principles to the non familiar students of various discipline of life sciences.
Summary
Course Status : Completed
Course Type : Core
Duration : 8 weeks
Category :
  • Biological Sciences & Bioengineering
Credit Points : 2
Level : Undergraduate
Start Date : 22 Jan 2020
End Date : 15 Mar 2020
Enrollment Ends : 08 Mar 2020
Exam Date : 15 Sep 2020 IST

Note: This exam date is subjected to change based on seat availability. You can check final exam date on your hall ticket.


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Course layout

Week- I

1. Revisiting the History and Concepts
2. Considering nutritional requirements
3. Introducing Morphogenesis 
        

Week - II

4. Identifying basic types of tissue culture
5. Identifying allied types of tissue culture techniques
6. Examinng the Structure of Nuclieic Acid


Week - III

7. Introducing DNA Processes 
8. Understanding the Basics of a Gene
9. DNA Sequencing methods


Week - IV

10. Exploring the fundamentals of a recombinant DNA
11. Examining the vector elements of DNA
12. Manipulating DNA enzymes


Week - V

13. Constructing recombinant DNA molecules
14. Designing plant transformation
15. Familiarizing with reporter genes and promoters


Week - VI

16. Analysing the methods of indirect gene transfer
17. Analyzing methods of direct gene transfer


Week - VII

18. Introducing the structure of antibodies
19. Identifying the structure of an antigen
20. Comparing antibody and antigen


Week - VIII

21. Familiarising with Monoclonal and Polyclonal antibodies
22. Applying hybridoma technology

Books and references

1. Lewin, B. (2004).  Genes VIII. Oxford University Press, Oxford. p.260

2. Goodwin, T. W and Mercer, E.I. 2003. Introduction to Plant Biochemistry (II edn.). CBS Publishers and Distributors, New Delhi. p.677.

3. Horton. H. R., Moran, L. A. , Ochs, R. S., Rawn J. D. and Scrimgeour, K. G. (1996). The Principles of Biochemistry (II edn). Prentice Hall International Inc., USA. p801. 

4. Twyman, R. M. (1999). Advanced Molecular biology. A concise Reference. Viva books private Limited, New Delhi. P.499.

5. Raineri, D. (2001). Introduction to Molecular Biology. Blackwell Science, Inc., p.190.

6. Malacinski, G.M. and Freifelder, D. (1998) Essential of Molecular Biology (IIIedn.). Jones and Bartlett Publishers, Inc., p. 531.

Instructor bio





Dr.P.Nagarajan

Tamilnadu Agricultural University, Tamil Nadu
Professor Pothi. Nagarajan, is basically a farm graduate with B.Sc., (Ag) degree and M.Sc., (Ag).in Plant Breeding and Genetics. He got his Ph.D. (Biotechnology) degree from the first set of Ph.D. (Biotechnology) at TNAU. He got his post doctoral training on Molecular Marker Assisted Breeding for Crop Plants at Texas A&M University, College Station, USA, sponsored by the Rockefeller Foundation, USA. His area of specialization is Molecular Breeding to evolve pest and disease resistant crop plants and biofortification of nutrients in crops utilizing molecular markers and bioinformatics tools. He served as the first Head of the Department of Plant Molecular Biology & Bioinformatics, TNAU. He an eminent teacher of Molecular Biology, published a book entitled "Molecular Biology - A Practical Approach". He is involved in teaching B. Tech., (Biotech.), B. Tech. (Bioinfo.), M.Sc., (Ag) and Ph.D. Plant Breeding and Genetics and Biotechnology, B.Sc. (Ag.) and B.Sc., (Hort.) and also involved in the formulation and revision of the syllabi for B. Tech., M.Sc., and Ph.D. courses of Biotechnology and Bioinformatics. He has served as the Chairman of the advisory committee of 25 M.Sc. and 15 Ph.D. students of Biotechnology, Bioinformatics, Plant Breeding and Genetics; member of the advisory committee of 20 Ph.D. students. He has secured several externally funded projects on molecular breeding and marker assisted selection for pest and disease resistance in crop plants with a total budget outlay of Rs. 326 lakhs from the Rockefeller foundation, USA; DBT, and DST, New Delhi. Expertise in AFLP protocol, he has designed a STS primer of an AFLP fragment closely linked to the WBPH resistance gene and identified WBPH resistance rice lines with higher yield. Tall/Dwarf specific RAPD, ISSR and SSR markers useful for juvenile selection of dwarf/tall coconuts in the nursery were identified by him. Laboratory screening protocol for MYMV was standardized and MYMV resistant mungbean lines identified by him were used as resistance donors in resistance breeding programmes. RGA primers based on NBS-LRR domain were also developed by him for the first time in mungbean. He was associated with fourteen AICRPs funded by ICAR on pulses, millets and oil seed crops and the release of the seven crop varieties viz., CO5 green gram, CO6 cowpea, CO6 red gram, CO2 soybean, ALR 2, ALR 3 groundnut and X7 cumbu. For the first time the seeds of ALR 2 have been deposited in long-term storage at the U.S. National seed Storage Lab., 1111 S. Mason St. Fort Collins, CO 80521-4500, USA. He has Published 126 research papers and popular articles in national and international journals. He is the coordinator of the Government of India, Department of Biotechnology, Biotechnology Information System-Distributed Information Sub Centre (DICs) of TNAU. He was associated with the preparation of Germplasm retrieval system (computerized catalogue) for red gram, cowpea and pearl millet. Served as the Information officer of the BTIS scheme. He has served as the Manager of the Madras Agricultural Journal. Life member in Society of Plant Biochemistry and Biotechnology. He has visited Quebec, Canada, to present paper at the International Congress on Plant Molecular Biology, organized by ISPMB; Phuket, Thailand, to attend and present a paper at the International Programme on Rice Biotechnology, organized by Rockefeller Foundation, New York.

Course certificate

30% for in course Assessment & 70% of end term Proctored Exam


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