Analytical Techniques

By Prof. M.R Rajeswari | Department of Biochemistry, All India Institute of Medical Sciences, New Delhi

Learners enrolled: 2265

The analytical techniques routinely used in Biochemistry take a very important place in Biochemistry, learning them is not just a requirement but, absolutely essential. This is because, the understanding of the subject mainly comes from the strong basis of the experiments and techniques on which the theories are built. This course deals with various techniques their principles, experimental details etc., including general Laboratory techniques, separation techniques based on hydrodynamic properties, centrifugation (sub-cellular fractionation etc), and Chromatographic, and Electrophortic techniques , HPLC, Purification methods, ELISA etc. Application in biomedical research and some clinical diagnostic methods RIA, ELISA, PET etc., Moleular diagnostics ,PCR, etc., Microscopic techniques and clinical biochemistry.

Summary

Course Status :	Completed
Course Type :	Core
Duration :	15 weeks
Category :	Biological Sciences & Bioengineering
Credit Points :	4
Level :	Postgraduate
Start Date :	01 Jul 2020
End Date :	14 Dec 2020
Enrollment Ends :	14 Oct 2020
Exam Date :

Page Visits

Course layout

Week 1

Aqueous solutions , Acids, Bases, buffers systems and pH meter,Colorimetry and UV-VIS absorption spectroscopy,Introduction to Hydrodynamic Techniques

Week 2

Centrifugation: Principles & Methodology,Density Gradient centrifugation : Rate Zonal and isopycnic,Differential centrifugation for Sub-cellular Fractionation

Week 3

Gel filtration: Principle, Methodology & Applications,Planar Chromatography: Principles and Applications

Week 4

Ion-exchange chromatography,Affinity chromatography

Week 5

Gas chromatography,HPLC,Protein Estimation Techniques

Week 6

Assessment-2,Light Microscopy and Confocal Microscopy,Fluorescence Microscopy : Application to live cell imaging

Week 7

IHC and IF,Flow cytometry: Theory and concept,Flow cytometry:Application in biology and medicine

Week 8

Electron Microscopy, TEM and SEM,SDS-PAGE

Week 9

Western Blotting Techniques,2D Electrophoresis and DIGE,Principles of Mass spectrometry

Week 10

Clinical Proteomics,Methods to quantify and Integrity check of DNA

Week 11

Methods to quantify and Integrity check of RNA,PCR and Real Time PCR,Blotting techniques for Nucleic acids

Week 12

Sequencing techniques of nucleic acids,Introduction to Recombinant DNA Technology,Assessment-2

Week 13

Expression techniques of recombinant proteins using Bacteria and yeast,Expression techniques of recombinnat proteins by insects and mammalian cells,Introduction to Genomic Techniques

Week 14

Techniques for studying Nucleic acid protein interactions,ELISA, RIA :Diagnostic applications

Week 15

CLIA and its Diagnostic applications,PET and its Diagnostic applications,Single cell biology,Final Examination

Instructor bio

Prof. M.R Rajeswari

Department of Biochemistry, All India Institute of Medical Sciences, New Delhi

Dr. MOGANTY R. RAJESWARI obtained her M.Sc. (Chem.) from Andhra University and M.Phil. and Ph.D. (Chem.) from University of Delhi. She has 30 years of teaching and 37 yrs of research experience. She supervised 44 thesis of Ph.D., M.D., M.D.S., M.Sc., M. Biotech. and M. Phil students as Chief- and co-supervisor and trained about 70 students from outside AIIMS She published more than 100 papers in International journals with high Impact Factors; written Reviews and authored two text books, “An Introduction to Biophysics” and “Studies on Carbapenemase and Membrane Proteome of Acinetobacter baumannii”;Chapters in text books of ‘Biochemistry”, NATO-published “Membrane biology” and e-book of NISCAIR-CSIR. She was the PI for developing E-Content for e-PG PATHSHALA under UGC-MHRD Project and National Coordinator for INDO–FRENCH Collaboration: between ICMR – INSERM programme on “TRE associated neuro disorders: “Fragile X, Friedreich’s Ataxia and other mental retardation”. Dr Rajeswari's major research interest has been 'Conformation of DNA, Protein and DNA-Protein Interactions in Health and Human Disease'. Her research interests include –a) the pathophysiology of unusual DNA structures in neurodegenerative disorders, FRDA, SCAs (2,3,12) which are associated with DNA repeat expansions and developing Biomarkers using Proteomics and nucleic acids ; b) Functional role of DNA binding HMG proteins in cancer progression and potential role as tumor markers ;c) application of DNA triple helix forming oligonucleotides as therapeutics in human disease. She received 18 Research grants from by DST, DBT, UGC and ICMR. She is the Organizing Secretary of: International Conference on “Advances in Molecular Mechanisms of Neurological Disorders” 2013;“One day Seminar on Integration of Teaching and Research in Biophysics at Graduate and Postgraduate Level”,2014 and International Conference on “Friedreich’s Ataxia and DNA Structure in Health and Diseases”, 2015 at AIIMS. Currently she is the National CoOrdinator for the UGC-MHRD -PG Pathshala to develop econtent at PG level for the subject Biophysics. She is currently is the National CoOrdinator for the UGC-MHRD -PG Pathshala to develop econtent at PG level for the subject Biophysics.