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Swayam Central

Analytical Techniques

By Dr. MOGANTY R. RAJESWARI   |   All India Institute of Medical Sciences, New Delhi-110029

The analytical techniques routinely used in Biochemistry take a very important place in Biochemistry, learning them is not just a requirement but, absolutely essential. This is because, the understanding of the subject mainly comes from the strong basis of the experiments and techniques on which the theories are built. This course deals with various techniques their principles, experimental details etc., including general Laboratory techniques, separation techniques based on hydrodynamic properties,  centrifugation (sub-cellular fractionation etc),  and  Chromatographic, and Electrophortic  techniques , HPLC,  Purification methods, ELISA etc. Application in biomedical research and some clinical diagnostic methods  RIA, ELISA, PET etc., Moleular diagnostics ,PCR, etc., Microscopic techniques and  clinical biochemistry.

Learners enrolled: 3541

SUMMARY

Course Status : Ongoing
Course Type : Core
Duration : 15 weeks
Start Date : 15 Jul 2019
End Date : 31 Oct 2019
Exam Date : 09 Nov 2019
Enrollment Ends : 10 Sep 2019
Category :
  • Biological Sciences & Bioengineering
  • Level : Postgraduate

    COURSE LAYOUT

    Week 1 
    Aqueous solutions , Acids, Bases, buffers systems and pH meter, Colorimetry and UV-VIS absorption spectroscopy Introduction to Hydrodynamic Techniques 
    Week 2 
    Centrifugation: Principles & Methodology Density Gradient centrifugation : Rate Zonal and isopycnic  Differential centrifugation for Sub-cellular Fractionation  
    Week 3 
    Gel filtration: Principle, Methodology & Applications  Planar Chromatography:  Principles and Applications
    Week 4 
    Ion-exchange chromatography, Affinity chromatography
    Week 5
    Gas chromatography, HPLC, Protein Estimation Techniques
    Week 6
    Electron Microscopy, TEM and SEM  Fluorescence Microscopy : Application to live cell imaging
    Week 7
    IHC and IF, Flow cytometry: Theory and concept Flow cytometry:Application in biology and medicine
    Week 8
    Light Microscopy and  Confocal Microscopy, SDS-PAGE 
    Week 9
    Western Blotting Techniques, 2D Electrophoresis and DIGE,  Principles of Mass spectrometry 
    Week 10
    Clinical Proteomics Methods to quantify and Integrity check of DNA  
    Week 11
    Methods to quantify and Integrity check of RNA,   PCR and Real Time PCR, Blotting techniques for Nucleic acids
    Week 12
    Sequencing techniques of nucleic acids, Introduction to Recombinant DNA Technology
    Week 13
    Expression techniques of recombinant proteins using Bacteria and yeast, Expression techniques of recombinnat proteins by insects and mammalian cells, Introduction to Genomic Techniques
    Week 14
    Techniques for studying Nucleic acid,Protein interactions, ELISA, RIA :Diagnostic applications
    Week 15
    CLIA and its Diagnostic application, PET and its Diagnostic applications, Single cell biology

    BOOKS AND REFERENCES

    INSTRUCTOR BIO

    Dr. MOGANTY R. RAJESWARI
    All India Institute of Medical Sciences, New Delhi-110029

    Dr. MOGANTY R. RAJESWARI obtained her M.Sc. (Chem.) from Andhra University and M.Phil. and Ph.D. (Chem.) from University of Delhi. She has 30 years of teaching and 37 yrs of research experience. She supervised 44 thesis of Ph.D., M.D., M.D.S., M.Sc., M. Biotech. & M. Phil students as Chief- & co-supervisor and trained about 70 students from outside AIIMS  She published more than 100 papers in International journals with high Impact Factors; written Reviews and authored two text books, “An Introduction to Biophysics” and  “Studies on Carbapenemase & Membrane Proteome of Acinetobacter baumannii”;Chapters in text books of ‘Biochemistry”, NATO-published “Membrane biology” and e-book of NISCAIR-CSIR. She was the PI  for developing E-Content for e-PG PATHSHALA under UGC-MHRD Project and National Coordinator for INDO–FRENCH  Collaboration: between ICMR – INSERM programme on “TRE associated neuro disorders: “Fragile X, Friedreich’s Ataxia and other mental retardation”.

    Dr Rajeswari's major research interest has been 'Conformation of DNA, Protein & DNA-Protein Interactions in Health & Human Disease'. Her research interests include –a) the pathophysiology of unusual DNA structures in neurodegenerative disorders, FRDA, SCAs (2,3,12) which are associated with  DNA repeat expansions and developing Biomarkers using Proteomics & nucleic acids ;  b) Functional role of DNA binding HMG proteins in cancer  progression and potential role as  tumor markers ;c)  application of DNA triple helix forming oligonucleotides as therapeutics in human disease.  She received 18 Research grants from by DST, DBT, UGC and ICMR. She is the Organizing Secretary of: International Conference on “Advances in Molecular Mechanisms of Neurological Disorders” 2013;“One day Seminar on Integration of Teaching and Research in Biophysics at Graduate & Postgraduate Level”,2014 and International Conference on “Friedreich’s Ataxia and DNA Structure in Health & Diseases”, 2015 at AIIMS.  Currently she is the National CoOrdinator for the UGC-MHRD -PG Pathshala to develop econtent at PG level for the subject  Biophysics.

    She is currently is the National CoOrdinator for the UGC-MHRD -PG Pathshala to develop econtent at PG level for the subject  Biophysics.

    COURSE CERTIFICATE

    30 Marks will be allocated for Internal Assessment and 70 Marks will be allocated for external proctored examination

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