Analytical Techniques

Week 1 

Aqueous solutions , Acids, Bases, buffers systems and pH meter, Colorimetry and UV-VIS absorption spectroscopy Introduction to Hydrodynamic Techniques 

Week 2 

Centrifugation: Principles & Methodology Density Gradient centrifugation : Rate Zonal and isopycnic  Differential centrifugation for Sub-cellular Fractionation  

Week 3 

Gel filtration: Principle, Methodology & Applications  Planar Chromatography:  Principles and Applications

Week 4 

Ion-exchange chromatography, Affinity chromatography

Week 5

Gas chromatography, HPLC, Protein Estimation Techniques

Week 6

Electron Microscopy, TEM and SEM  Fluorescence Microscopy : Application to live cell imaging

Week 7

IHC and IF, Flow cytometry: Theory and concept Flow cytometry:Application in biology and medicine

Week 8

Light Microscopy and  Confocal Microscopy, SDS-PAGE 

Week 9

Western Blotting Techniques, 2D Electrophoresis and DIGE,  Principles of Mass spectrometry 

Week 10

Clinical Proteomics Methods to quantify and Integrity check of DNA  

Week 11

Methods to quantify and Integrity check of RNA,   PCR and Real Time PCR, Blotting techniques for Nucleic acids

Week 12

Sequencing techniques of nucleic acids, Introduction to Recombinant DNA Technology

Week 13

Expression techniques of recombinant proteins using Bacteria and yeast, Expression techniques of recombinnat proteins by insects and mammalian cells, Introduction to Genomic Techniques

Week 14

Techniques for studying Nucleic acid,Protein interactions, ELISA, RIA :Diagnostic applications

Week 15

CLIA and its Diagnostic application, PET and its Diagnostic applications, Single cell biology

Dr. MOGANTY R. RAJESWARI obtained her M.Sc. (Chem.) from Andhra University and M.Phil. and Ph.D. (Chem.) from University of Delhi. She has 30 years of teaching and 37 yrs of research experience. She supervised 44 thesis of Ph.D., M.D., M.D.S., M.Sc., M. Biotech. & M. Phil students as Chief- & co-supervisor and trained about 70 students from outside AIIMS  She published more than 100 papers in International journals with high Impact Factors; written Reviews and authored two text books, “An Introduction to Biophysics” and  “Studies on Carbapenemase & Membrane Proteome of Acinetobacter baumannii”;Chapters in text books of ‘Biochemistry”, NATO-published “Membrane biology” and e-book of NISCAIR-CSIR. She was the PI  for developing E-Content for e-PG PATHSHALA under UGC-MHRD Project and National Coordinator for INDO–FRENCH  Collaboration: between ICMR – INSERM programme on “TRE associated neuro disorders: “Fragile X, Friedreich’s Ataxia and other mental retardation”.

Dr Rajeswari's major research interest has been 'Conformation of DNA, Protein & DNA-Protein Interactions in Health & Human Disease'. Her research interests include –a) the pathophysiology of unusual DNA structures in neurodegenerative disorders, FRDA, SCAs (2,3,12) which are associated with  DNA repeat expansions and developing Biomarkers using Proteomics & nucleic acids ;  b) Functional role of DNA binding HMG proteins in cancer  progression and potential role as  tumor markers ;c)  application of DNA triple helix forming oligonucleotides as therapeutics in human disease.  She received 18 Research grants from by DST, DBT, UGC and ICMR. She is the Organizing Secretary of: International Conference on “Advances in Molecular Mechanisms of Neurological Disorders” 2013;“One day Seminar on Integration of Teaching and Research in Biophysics at Graduate & Postgraduate Level”,2014 and International Conference on “Friedreich’s Ataxia and DNA Structure in Health & Diseases”, 2015 at AIIMS.  Currently she is the National CoOrdinator for the UGC-MHRD -PG Pathshala to develop econtent at PG level for the subject  Biophysics.

She is currently is the National CoOrdinator for the UGC-MHRD -PG Pathshala to develop econtent at PG level for the subject  Biophysics.